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ACCRNA Extraction Kit

ACCRNA Extraction kit is Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast,fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to  microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Description

 

ACCRNA EXTRACTION Kit

 

Reagents Provided   RTN10

10 Preps

RTN70

70 Preps

RTN350

350 Preps

Lysis Solution   10 mL 70 mL 350 mL
2-Mercaptoethanol (2-ME)   0.15 mL 0.9 mL 2 × 2 mL
Wash Solution A   6 mL 40 mL 200 mL
Wash Solution B   2.5 mL 15 mL 75 mL
Elution buffer   1.5 mL 10 mL 50 mL
Binding Columns   10 each 70 each 5 × 70 each
Collection Tubes, 2.0 mL capacity   4 × 10 each 4 × 70 each 20 × 70 each

 

Reagents and Equipment Required But Not Provided

  • Ethanol (200 proof )
  • 70% Ethanol solution
  • Proteinase K, Cat
  • 55 °C Water bath or heating block
  • Molecular Biology Reagent Water,
  • RNase-free pipette tips
  • Microcentrifuge tubes
  • Microcentrifuge
  • Column DNase I Digestion Set
  • Amplification Grade DNase I
  • RNAlater™, RNA Stabilization Solution for Tissue
  • Tissue homogenizer and vessels for tissue samples

    Storage and Stability

    Store the Kit at room temperature. If any reagent forms a precipitate, warm at 65 °C until the precipitate dissolves and allow to cool to room temperature before use. All reagents are stable for at least 1 year when stored properly.

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